Human Transcription factor SOX 10 (SOX10) ELISA Kit | SOX10 elisa kit

Human Transcription factor SOX 10 (SOX10) ELISA Kit

Cat. Num. AAA7244823

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Human
• Synonyms: Transcription factor SOX 10 (SOX10); Human Transcription factor SOX 10 (SOX10) ELISA Kit; SOX10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOX10. No significant cross-reactivity or interference between SOX10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SOX10 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOX10 elisa kit

Intended Uses: This SOX10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SOX10. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: SOX10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SOX10 antibody and an SOX10-HRP conjugate. The assay sample and buffer are incubated together with SOX10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOX10 concentration since SOX10 from samples and SOX10-HRP conjugate compete for the anti-SOX10 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOX10 from the sample, fewer sites are left to bind SOX10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOX10 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SOX10 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872.1
• NCBI GenBank Nucleotide #: NM_006941.3
• Molecular Weight: 31,088 Da
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY (sex determining region Y)-box 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10; SRY-related HMG-box gene 10; dominant megacolon, mouse, human homolog of
• UniProt Protein Name: Transcription factor SOX-10
• Protein Family: Transcription factor
• UniProt Gene Name: SOX10
• UniProt Entry Name: SOX10_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extrinsic to mitochondrial outer membrane; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; protein binding; transcription coactivator activity; chromatin binding; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of neuroblast proliferation; developmental growth; anatomical structure morphogenesis; positive regulation of gliogenesis; in utero embryonic development; cell maturation; digestive tract morphogenesis; enteric nervous system development; peripheral nervous system development; regulation of transcription from RNA polymerase II promoter; melanocyte differentiation; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural crest cell migration; negative regulation of apoptosis

Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 2e; Waardenburg Syndrome, Type 4c