Chicken Cytotoxic and regulatory T-cell molecule (CRTAM) ELISA Kit | CRTAM elisa kit

Chicken Cytotoxic and regulatory T-cell molecule (CRTAM) ELISA Kit

Cat. Num. AAA7238963

• Gene Names: CRTAM; CD355
• Reactivity: Chicken
• Synonyms: Cytotoxic and regulatory T-cell molecule (CRTAM); Chicken Cytotoxic and regulatory T-cell molecule (CRTAM) ELISA Kit; CRTAM elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRTAM. No significant cross-reactivity or interference between CRTAM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRTAM and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRTAM elisa kit

Intended Uses: This CRTAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CRTAM. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CRTAM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CRTAM antibody and an CRTAM-HRP conjugate. The assay sample and buffer are incubated together with CRTAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRTAM concentration since CRTAM from samples and CRTAM-HRP conjugate compete for the anti-CRTAM antibody binding site. Since the number of sites is limited, as more sites are occupied by CRTAM from the sample, fewer sites are left to bind CRTAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRTAM concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51593098
• NCBI GeneID: 56253
• NCBI Accession #: NP_062550.2
• NCBI GenBank Nucleotide #: NM_019604.2
• Molecular Weight: 22,256 Da
• NCBI Official Full Name: cytotoxic and regulatory T-cell molecule
• NCBI Official Synonym Full Names: cytotoxic and regulatory T cell molecule
• NCBI Official Symbol: CRTAM
• NCBI Official Synonym Symbols: CD355
• NCBI Protein Information: cytotoxic and regulatory T-cell molecule; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; class-I MHC-restricted T-cell-associated molecule
• UniProt Protein Name: Cytotoxic and regulatory T-cell molecule
• Protein Family: Cytotoxic and regulatory T-cell molecule
• UniProt Gene Name: CRTAM
• UniProt Entry Name: CRTAM_HUMAN

NCBI Description

The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al., 2008 [PubMed 18329370]).[supplied by OMIM, Feb 2009]

Uniprot Description

CRTAM: Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: receptor binding

Biological Process: regulation of immune response; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; detection of tumor cell; activated T cell proliferation; detection of stimulus; cell recognition; positive regulation of natural killer cell mediated cytotoxicity; T cell mediated cytotoxicity; positive regulation of cytokine secretion