Typical Standard Curve/Testing Data
Rat Cytoskeleton-associated protein 5 (CKAP5) ELISA Kit | CKAP5 elisa kit
Rat Cytoskeleton-associated protein 5 (CKAP5) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: CKAP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CKAP5 antibody and an CKAP5-HRP conjugate. The assay sample and buffer are incubated together with CKAP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKAP5 concentration since CKAP5 from samples and CKAP5-HRP conjugate compete for the anti-CKAP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CKAP5 from the sample, fewer sites are left to bind CKAP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKAP5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Uniprot Description
CKAP5: Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. Belongs to the TOG/XMAP215 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: microtubule cytoskeleton; spindle pole; centrosome; protein complex; membrane; cytosol; gamma-tubulin complex
Molecular Function: protein binding
Biological Process: mitosis; centrosome organization and biogenesis; cell division; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; spindle organization and biogenesis; RNA transport; establishment and/or maintenance of microtubule cytoskeleton polarity
Research Articles on CKAP5
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Product Notes
The Rat CKAP5 ckap5 (Catalog #AAA7235007) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235007 ELISA Kit recognizes Rat CKAP5. It is sometimes possible for the material contained within the vial of "Cytoskeleton-associated protein 5 (CKAP5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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