Typical Testing Data/Standard Curve (for reference only)
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Human Brother of CDO (BOC) ELISA Kit | BOC elisa kit
Human Brother of CDO (BOC) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: BOC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BOC antibody and an BOC-HRP conjugate. The assay sample and buffer are incubated together with BOC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BOC concentration since BOC from samples and BOC-HRP conjugate compete for the anti-BOC antibody binding site. Since the number of sites is limited, as more sites are occupied by BOC from the sample, fewer sites are left to bind BOC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BOC concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the immunoglobulin/fibronectin type III repeat family. It is a component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells, and promotes myogenic differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
Uniprot Description
BOC: Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell development/differentiation; Cell adhesion
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: cell soma; integral to membrane; plasma membrane
Molecular Function: protein binding
Biological Process: axon guidance; smoothened signaling pathway; muscle cell differentiation; positive regulation of muscle cell differentiation; cell adhesion; positive regulation of myoblast differentiation
Research Articles on BOC
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Product Notes
The Human BOC boc (Catalog #AAA7234550) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234550 ELISA Kit recognizes Human BOC. It is sometimes possible for the material contained within the vial of "Brother of CDO (BOC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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