Related Product Information for ES elisa kit
For samples:
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
1. INTENDED USE
This ES ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative
determination of Mouse ES. This ELISA kit for research use only, not for therapeutic
or applications!
2. PRINCIPLE OF THE ASSAY
ES ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The
microtiter plate has been pre-coated with a monoclonal antibody specific for ES.
Standards or samples are then added to the microtiter plate wells and ES if present,
will bind to the antibody pre-coated wells. In order to quantitatively determine the
amount of ES present in the sample, a standardized preparation of horseradish
peroxidase (HRP)-conjugated polyclonal antibody, specific for ES are added to each
well to “sandwich” the ES immobilized on the plate. The microtiter plate undergoes
incubation, and then the wells are thoroughly washed to remove all unbound
components. Next, substrate solutions are added to each well. The enzyme (HRP) and
substrate are allowed to react over a short incubation period. Only those wells that
contain ES and enzyme-conjugated antibody will exhibit a change in color. The
enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm. A
standard curve is plotted relating the intensity of the color (O.D.) to the concentration
of standards. The ES concentration in each sample is interpolated from this standard
curve.
Product Categories/Family for ES elisa kit
Product Notes
The Mouse ES (Catalog #
AAA723362) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723362 ELISA Kit recognizes Mouse ES.
It is sometimes possible for the material contained within the vial of
"Endostatin, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
cannot be held
responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
any time and without notice. It is the responsibility of the customer to inform AAA Biotech
of any product performance
issues observed or experienced within 30 days of receipt of said product. To see additional
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