Typical Standard Curve/Testing Data
Human Cysteine protease ATG4C (ATG4C) ELISA Kit | ATG4C elisa kit
Human Cysteine protease ATG4C (ATG4C) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: ATG4C ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATG4C antibody and an ATG4C-HRP conjugate. The assay sample and buffer are incubated together with ATG4C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG4C concentration since ATG4C from samples and ATG4C-HRP conjugate compete for the anti-ATG4C antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG4C from the sample, fewer sites are left to bind ATG4C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG4C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family.
Protein type: Autophagy; EC 3.4.22.-; Protease; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: cytoplasm; extracellular region; cytosol
Molecular Function: peptidase activity; cysteine-type endopeptidase activity
Biological Process: protein delipidation; mitochondrion degradation; autophagy; C-terminal protein lipidation; protein processing; proteolysis; protein targeting to membrane; cellular response to nitrogen starvation; autophagic vacuole formation
Research Articles on ATG4C
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Product Notes
The Human ATG4C atg4c (Catalog #AAA7233215) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233215 ELISA Kit recognizes Human ATG4C. It is sometimes possible for the material contained within the vial of "Cysteine protease ATG4C (ATG4C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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