Porcine Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) ELISA Kit | ALOX5AP elisa kit

Porcine Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) ELISA Kit

Cat. Num. AAA7232829

• Gene Names: ALOX5AP; FLAP
• Reactivity: Porcine
• Synonyms: Arachidonate 5-lipoxygenase-activating protein (ALOX5AP); Porcine Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) ELISA Kit; ALOX5AP elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of ALOX5AP. No significant cross-reactivity or interference between ALOX5AP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALOX5AP and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOX5AP elisa kit

Intended Uses: This ALOX5AP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ALOX5AP. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ALOX5AP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALOX5AP antibody and an ALOX5AP-HRP conjugate. The assay sample and buffer are incubated together with ALOX5AP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX5AP concentration since ALOX5AP from samples and ALOX5AP-HRP conjugate compete for the anti-ALOX5AP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX5AP from the sample, fewer sites are left to bind ALOX5AP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX5AP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119628872
• NCBI GeneID: 241
• Molecular Weight: 18,157 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase-activating protein
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase-activating protein
• NCBI Official Symbol: ALOX5AP
• NCBI Official Synonym Symbols: FLAP
• NCBI Protein Information: arachidonate 5-lipoxygenase-activating protein; MK-886-binding protein
• UniProt Protein Name: Arachidonate 5-lipoxygenase-activating protein
• Protein Family: Arachidonate 5-lipoxygenase-activating protein
• UniProt Gene Name: ALOX5AP
• UniProt Synonym Gene Names: FLAP
• UniProt Entry Name: AL5AP_HUMAN

NCBI Description

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Nuclear envelope; Activator; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: arachidonic acid binding; protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; protein N-terminus binding; glutathione peroxidase activity

Biological Process: positive regulation of catalytic activity; leukotriene biosynthetic process; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Stroke, Ischemic