Mouse Cell division cycle protein 123 homolog (CDC123) ELISA Kit | CDC123 elisa kit
Mouse Cell division cycle protein 123 homolog (CDC123) ELISA Kit
Principle of the Assay: CDC123 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC123 antibody and an CDC123-HRP conjugate. The assay sample and buffer are incubated together with CDC123-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theCDC123 concentration since CDC123 from samples and CDC123-HRP conjugate compete for the anti-CDC123 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC123 from the sample, fewer sites are left to bind CDC123-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC123 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CDC123: Required for S phase entry of the cell cycle. Belongs to the CDC123 family.
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: cytoplasm
Biological Process: cell division; positive regulation of cell proliferation; regulation of mitotic cell cycle; cell cycle arrest