Typical Testing Data/Standard Curve (for reference only)
Human Apoptosis associated speck like protein containing a CARD (PYCARD) ELISA Kit | PYCARD elisa kit
Human Apoptosis associated speck like protein containing a CARD (PYCARD) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PYCARD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PYCARD antibody and an PYCARD-HRP conjugate. The assay sample and buffer are incubated together with PYCARD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYCARD concentration since PYCARD from samples and PYCARD-HRP conjugate compete for the anti-PYCARD antibody binding site. Since the number of sites is limited, as more sites are occupied by PYCARD from the sample, fewer sites are left to bind PYCARD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYCARD concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Tumor suppressor
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: mitochondrion; cell soma; endoplasmic reticulum; cytoplasm; nucleolus; extracellular region; IkappaB kinase complex; cytosol; nucleus
Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding
Biological Process: apoptosis; positive regulation of apoptosis; myeloid dendritic cell activation during immune response; positive regulation of JNK cascade; positive regulation of caspase activity; regulation of protein stability; positive regulation of interleukin-1 beta secretion; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; signal transduction; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; regulation of autophagy; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II
Research Articles on PYCARD
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Product Notes
The Human PYCARD pycard (Catalog #AAA7227989) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227989 ELISA Kit recognizes Human PYCARD. It is sometimes possible for the material contained within the vial of "Apoptosis associated speck like protein containing a CARD (PYCARD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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