Typical Testing Data/Standard Curve (for reference only)
Human ADP dependent glucokinase (ADPGK) ELISA Kit | ADPGK elisa kit
Human ADP dependent glucokinase (ADPGK) ELISA Kit
Gene Names
ADPGK; ADP-GK; 2610017G09Rik
Reactivity
Human
Synonyms
ADP dependent glucokinase (ADPGK); Human ADP dependent glucokinase (ADPGK) ELISA Kit; ADPGK elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ADPGK elisa kit
Intended Uses: This ADP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ADP. This ELISA kit for research use only!
Principle of the Assay: ADP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADP antibody and an ADP-HRP conjugate. The assay sample and buffer are incubated together with ADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADP concentration since ADP from samples and ADP-HRP conjugate compete for the anti-ADP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADP from the sample, fewer sites are left to bind ADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ADP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADP antibody and an ADP-HRP conjugate. The assay sample and buffer are incubated together with ADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADP concentration since ADP from samples and ADP-HRP conjugate compete for the anti-ADP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADP from the sample, fewer sites are left to bind ADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
8,252 Da
NCBI Official Full Name
ADP-dependent glucokinase
NCBI Official Synonym Full Names
ADP-dependent glucokinase
NCBI Official Symbol
ADPGK
NCBI Official Synonym Symbols
ADP-GK; 2610017G09Rik
NCBI Protein Information
ADP-dependent glucokinase; rbBP-35; ATP-dependent glucokinase
UniProt Protein Name
ADP-dependent glucokinase
Protein Family
UniProt Gene Name
ADPGK
UniProt Synonym Gene Names
PSEC0260; ADP-GK; ADPGK
UniProt Entry Name
ADPGK_HUMAN
NCBI Description
ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008]
Uniprot Description
ADPGK: Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency. Belongs to the ADP-dependent glucokinase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.147; Secreted, signal peptide; Kinase, other; Secreted
Chromosomal Location of Human Ortholog: 15q24.1
Cellular Component: membrane; extracellular region
Molecular Function: ADP-specific glucokinase activity; metal ion binding
Biological Process: glycolysis; phosphorylation
Research Articles on ADPGK
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Product Notes
The Human ADPGK adpgk (Catalog #AAA7223816) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7223816 ELISA Kit recognizes Human ADPGK. It is sometimes possible for the material contained within the vial of "ADP dependent glucokinase (ADPGK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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