Rat Activity-regulated cytoskeleton-associated protein (ARC) ELISA Kit | ARC elisa kit

Rat Activity-regulated cytoskeleton-associated protein (ARC) ELISA Kit

Cat. Num. AAA7219950

• Gene Names: ARC; Arg3.1
• Reactivity: Rat
• Synonyms: Activity-regulated cytoskeleton-associated protein (ARC); Rat Activity-regulated cytoskeleton-associated protein (ARC) ELISA Kit; ARC elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This ARC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ARC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARC elisa kit

Principle of the assay: ARC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARC antibody and an ARC-HRP conjugate. The assay sample and buffer are incubated together with ARC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARC concentration since ARC from samples and ARC-HRP conjugate compete for the anti-ARC antibody binding site. Since the number of sites is limited, as more sites are occupied by ARC from the sample, fewer sites are left to bind ARC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ARC elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 8567330
• NCBI GeneID: 23237
• NCBI Accession #: NP_056008.1
• NCBI GenBank Nucleotide #: NM_015193.4
• Molecular Weight: 45,316 Da
• NCBI Official Full Name: activity-regulated cytoskeleton-associated protein
• NCBI Official Synonym Full Names: activity-regulated cytoskeleton-associated protein
• NCBI Official Symbol: ARC
• NCBI Official Synonym Symbols: Arg3.1
• NCBI Protein Information: activity-regulated cytoskeleton-associated protein; ARC/ARG3.1; activity-regulated gene 3.1 protein homolog
• UniProt Protein Name: Activity-regulated cytoskeleton-associated protein
• Protein Family: AAA ATPase forming ring-shaped complexes
• UniProt Gene Name: ARC
• UniProt Synonym Gene Names: KIAA0278; Arg3.1
• UniProt Entry Name: ARC_HUMAN

Uniprot Description

ARG3.1: Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. Belongs to the ARC/ARG3.1 family.

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: postsynaptic membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic spine; acrosome; cell junction; endosome; actin cytoskeleton

Biological Process: anterior/posterior pattern formation; cell migration; regulation of cell morphogenesis; endocytosis; cytoskeleton organization and biogenesis; learning; endoderm development; regulation of neuronal synaptic plasticity