Typical Testing Data/Standard Curve (for reference only)
Rat Yorkie homolog (YAP1) ELISA Kit | YAP1 elisa kit
Rat Yorkie homolog (YAP1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: YAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-YAP1 antibody and an YAP1-HRP conjugate. The assay sample and buffer are incubated together with YAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YAP1 concentration since YAP1 from samples and YAP1-HRP conjugate compete for the anti-YAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by YAP1 from the sample, fewer sites are left to bind YAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YAP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
Uniprot Description
YAP1: an adaptor protein that binds to HER4 and the SH3 domain of the Yes tyrosine kinase. Associates with multiple transcription factors in the nucleus, and appears to be a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus. Contains a WW domain that is found in various structural, regulatory and signaling molecules.
Protein type: Transcription, coactivator/corepressor; Oncoprotein
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; transcription factor complex; membrane; cytoplasm; cytosol; nucleus; cell junction
Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription corepressor activity
Biological Process: paraxial mesoderm development; transcription initiation from RNA polymerase II promoter; somatic stem cell maintenance; lateral mesoderm development; cell morphogenesis; negative regulation of epithelial cell differentiation; notochord development; positive regulation of organ growth; keratinocyte differentiation; cell proliferation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; gene expression; vasculogenesis; response to DNA damage stimulus
Disease: Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/palate, And/or Mental Retardation
Research Articles on YAP1
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Product Notes
The Rat YAP1 yap1 (Catalog #AAA7219336) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7219336 ELISA Kit recognizes Rat YAP1. It is sometimes possible for the material contained within the vial of "Yorkie homolog (YAP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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