Typical Testing Data/Standard Curve (for reference only)
Mouse Calponin-1 (CNN1) ELISA Kit | CNN1 elisa kit
Mouse Calponin-1 (CNN1) ELISA Kit
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Mouse
Synonyms
Calponin-1 (CNN1); Mouse Calponin-1 (CNN1) ELISA Kit; CNN1 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This CNN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CNN1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CNN1 elisa kit
Principle of the assay: CNN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNN1 antibody and an CNN1-HRP conjugate. The assay sample and buffer are incubated together with CNN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNN1 concentration since CNN1 from samples and CNN1-HRP conjugate compete for the anti-CNN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNN1 from the sample, fewer sites are left to bind CNN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNN1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CNN1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,170 Da
NCBI Official Full Name
calponin-1
NCBI Official Synonym Full Names
calponin 1, basic, smooth muscle
NCBI Official Symbol
CNN1
NCBI Official Synonym Symbols
SMCC; Sm-Calp; HEL-S-14
NCBI Protein Information
calponin-1; basic calponin; calponins, basic; calponin H1, smooth muscle; epididymis secretory protein Li 14
UniProt Protein Name
Calponin-1
Protein Family
UniProt Gene Name
CNN1
UniProt Entry Name
CNN1_HUMAN
Uniprot Description
calponin 1: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.
Protein type: Contractile; Actin-binding; Myosin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19p13.2-p13.1
Cellular Component: focal adhesion; cytoskeleton
Molecular Function: calmodulin binding; actin binding
Biological Process: regulation of smooth muscle contraction; actomyosin structure organization and biogenesis
Research Articles on CNN1
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Product Notes
The Mouse CNN1 cnn1 (Catalog #AAA7214739) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214739 ELISA Kit recognizes Mouse CNN1. It is sometimes possible for the material contained within the vial of "Calponin-1 (CNN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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