Human Hexokinase 3 ELISA Kit | HK3 elisa kit

Human Hexokinase 3 ELISA Kit

Cat. Num. AAA721045

• Gene Names: HK3; HXK3; HKIII
• Reactivity: Human
• Synonyms: Hexokinase 3; Human Hexokinase 3 ELISA Kit; HK3 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HK3 elisa kit

Intended Uses: This HK3 ELISA kit is intended Laboratory for research use only. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHK3 in the sample, thisHK3 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHK3 concentration. The concentration ofHK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This HK3 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHK3. Standards or samples are then added to the microtiter plate wells andHK3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHK3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHK3 are added to each well to "sandwich" theHK3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that containHK3 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for HK3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 194097330
• NCBI GeneID: 3101
• NCBI Accession #: NP_002106.2
• NCBI GenBank Nucleotide #: NM_002115.2
• Molecular Weight: 99,025 Da
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3 (white cell)
• NCBI Official Symbol: HK3
• NCBI Official Synonym Symbols: HXK3; HKIII
• NCBI Protein Information: hexokinase-3; HK III; hexokinase type III; ATP:D-hexose 6-phosphotransferase
• UniProt Protein Name: Hexokinase-3
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK3
• UniProt Synonym Gene Names: HK III
• UniProt Entry Name: HXK3_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: EC 2.7.1.1; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Enzyme, cellular metabolism; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Transferase

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: hexokinase activity; enzyme binding; hormone binding; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport