Typical Standard Curve/Testing Data
Rat Protamine 1 ELISA Kit | PRM 1 elisa kit
Rat Protamine 1 ELISA Kit
Reactivity
Rat
Synonyms
Protamine 1; Rat Protamine 1 ELISA Kit; PRM 1 elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
25-500 pg/mL
Sensitivity
1.0pg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for PRM 1 elisa kit
Intended Uses: This PRM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PRM1. This ELISA kit for research use only!
Principle of the Assay: PRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRM1 antibody and an PRM1-HRP conjugate. The assay sample and buffer are incubated together with PRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRM1 concentration since PRM1 from samples and PRM1-HRP conjugate compete for the anti-PRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRM1 from the sample, fewer sites are left to bind PRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRM1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRM1 antibody and an PRM1-HRP conjugate. The assay sample and buffer are incubated together with PRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRM1 concentration since PRM1 from samples and PRM1-HRP conjugate compete for the anti-PRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRM1 from the sample, fewer sites are left to bind PRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRM1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PRM 1 elisa kit
NCBI and Uniprot Product Information
Uniprot Description
PRM1: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. Belongs to the protamine P1 family.
Protein type: Cancer Testis Antigen (CTA); DNA-binding
Chromosomal Location of Human Ortholog: 16p13.2
Cellular Component: nucleoplasm; nucleosome
Molecular Function: DNA binding
Biological Process: chromosome condensation; multicellular organismal development; spermatogenesis; cell differentiation; DNA packaging
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Product Notes
The Rat PRM 1 prm1 (Catalog #AAA720976) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720976 ELISA Kit recognizes Rat PRM 1. It is sometimes possible for the material contained within the vial of "Protamine 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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