Human Cyclin O (CCNO) ELISA Kit | CCNO elisa kit

Human Cyclin O (CCNO) ELISA Kit

Cat. Num. AAA7208864

• Gene Names: CCNO; CCNU; UDG2; CILD29
• Reactivity: Human
• Synonyms: Cyclin O (CCNO); Human Cyclin O (CCNO) ELISA Kit; CCNO elisa kit

• Reactivity: Human

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCNO elisa kit

Intended Uses: This CCNO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCNO. This ELISA kit for research use only!

Principle of the Assay: CCNO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CCNO antibody and an CCNO-HRP conjugate. The assay sample and ‘buffer are incubated together with CCNO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCNO concentration since CCNO from samples and CCNO-HRP conjugate compete for the anti-CCNO antibody binding site. Since the number of sites is limited, as more sites are occupied by CCNO from the sample, fewer sites are left to bind CCNO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCNO concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CCNO elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 153791755
• NCBI GeneID: 10309
• NCBI Accession #: NP_066970.3
• NCBI GenBank Nucleotide #: NM_021147.4
• Molecular Weight: 14,297 Da
• NCBI Official Full Name: cyclin-O
• NCBI Official Synonym Full Names: cyclin O
• NCBI Official Symbol: CCNO
• NCBI Official Synonym Symbols: CCNU; UDG2; CILD29
• NCBI Protein Information: cyclin-O; cyclin U; cyclin domain containing
• UniProt Protein Name: Cyclin-O
• Protein Family: Cyclin
• UniProt Gene Name: CCNO
• UniProt Entry Name: CCNO_HUMAN

NCBI Description

This gene encodes a member of the cyclin protein family, and the encoded protein is involved in regulation of the cell cycle. Disruption of this gene is associated with primary ciliary dyskinesia-19. Alternative splicing results in multiple transcript variants. This gene, which has a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase in PubMed ID: 2001396. A later publication, PubMed ID: 8419333, identified this gene's product as a cyclin protein family member. The UNG2 symbol is also used as a specific protein isoform name for the UNG gene (GeneID 7374), so confusion exists in the scientific literature and in some databases for these two genes. [provided by RefSeq, Jul 2014]

Uniprot Description

CCNO: This gene, which has a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase in PubMed ID: 2001396. A later publication, PubMed ID: 8419333, identified this gene's product as a cyclin protein family member. The UNG2 term is also used as a specific protein isoform name for the UNG gene (GeneID 7374), so confusion exists in the scientific literature and in some databases for these two genes.[provided by RefSeq, Nov 2010]

Protein type: Hydrolase; EC 3.2.2.-; Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: uracil DNA N-glycosylase activity

Biological Process: response to drug; cell division; base-excision repair; cilium biogenesis; cell cycle

Disease: Ciliary Dyskinesia, Primary, 29