Guinea Pig Bone Morphogenetic Protein 7 ELISA Kit | BMP7 elisa kit

Guinea pig Bone Morphogenetic Protein 7 ELISA Kit

Cat. Num. AAA720855

• Gene Names: BMP7; OP-1
• Reactivity: Guinea Pig
• Synonyms: Bone Morphogenetic Protein 7; Guinea pig Bone Morphogenetic Protein 7 ELISA Kit; BMP7 elisa kit

• Reactivity: Guinea Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of BMP7. No significant cross-reactivity or interference between BMP7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMP7 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP7 elisa kit

Intended Uses: This BMP7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig BMP7. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: BMP7 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BMP7 antibody and an BMP7-HRP conjugate. The assay sample and buffer are incubated together with BMP7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP7 concentration since BMP7 from samples and BMP7-HRP conjugate compete for the anti-BMP7 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP7 from the sample, fewer sites are left to bind BMP7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BMP7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4502427
• NCBI GeneID: 655
• NCBI Accession #: NP_001710.1
• NCBI GenBank Nucleotide #: NM_001719.2
• Molecular Weight: 49,313 Da
• NCBI Official Full Name: bone morphogenetic protein 7
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7; osteogenic protein 1
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP7
• UniProt Synonym Gene Names: OP1; BMP-7; OP-1
• UniProt Entry Name: BMP7_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: response to peptide hormone stimulus; axon guidance; negative regulation of MAP kinase activity; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; embryonic pattern specification; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; BMP signaling pathway; response to vitamin D; epithelial cell differentiation; ureteric bud development; dendrite development; mesonephros development; skeletal development; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; mesenchymal cell differentiation; branching morphogenesis of a tube; mesoderm formation; inhibition of NF-kappaB transcription factor; cartilage development; negative regulation of mitosis; negative regulation of phosphorylation; epithelial to mesenchymal transition; negative regulation of Notch signaling pathway; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; metanephros development; neurite morphogenesis; growth