Human PRA1 family protein 3 (ARL6IP5) ELISA Kit | ARL6IP5 elisa kit

Human PRA1 family protein 3 (ARL6IP5) ELISA Kit

Cat. Num. AAA7207293

• Gene Names: ARL6IP5; JWA; jmx; hp22; PRAF3; DERP11; HSPC127; addicsin; GTRAP3-18
• Reactivity: Human
• Synonyms: PRA1 family protein 3 (ARL6IP5); Human PRA1 family protein 3 (ARL6IP5) ELISA Kit; ARL6IP5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ARL6IP5. No significant cross-reactivity or interference between ARL6IP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARL6IP5 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARL6IP5 elisa kit

Intended Uses: This ARL6IP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanARL6IP5. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ARL6IP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ARL6IP5 antibody and an ARL6IP5-HRP conjugate. The assay sample and buffer are incubated together with ARL6IP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARL6IP5 concentration since ARL6IP5 from samples and ARL6IP5-HRP conjugate compete for the anti-ARL6IP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARL6IP5 from the sample, fewer sites are left to bind ARL6IP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARL6IP5 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5453704
• NCBI GeneID: 10550
• NCBI Accession #: NP_006398.1
• NCBI GenBank Nucleotide #: NM_006407.3
• Molecular Weight: 21,615 Da
• NCBI Official Full Name: PRA1 family protein 3
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 6 interacting protein 5
• NCBI Official Symbol: ARL6IP5
• NCBI Official Synonym Symbols: JWA; jmx; hp22; PRAF3; DERP11; HSPC127; addicsin; GTRAP3-18
• NCBI Protein Information: PRA1 family protein 3; JM5; aip-5; protein JWa; PRA1 domain family 3; ARL-6-interacting protein 5; dermal papilla derived protein 11; dermal papilla-derived protein 11; prenylated Rab acceptor protein 2; putative MAPK activating protein PM27; putative MAPK-activating protein PM27; glutamate transporter EEAC1-associated protein; glutamate transporter EAAC1-interacting protein; cytoskeleton related vitamin A responsive protein; cytoskeleton-related vitamin A-responsive protein; ADP-ribosylation-like factor 6 interacting protein 5; ADP-ribosylation factor-like protein 6-interacting protein 5
• UniProt Protein Name: PRA1 family protein 3
• Protein Family: PRA1 family protein
• UniProt Gene Name: ARL6IP5
• UniProt Synonym Gene Names: DERP11; JWA; PRA2; PRAF3; HSPC127; ARL-6-interacting protein 5; Aip-5
• UniProt Entry Name: PRAF3_HUMAN

NCBI Description

Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL6IP5: Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic. Belongs to the PRA1 family.

Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 3p14

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein C-terminus binding

Biological Process: L-glutamate transport; positive regulation of apoptosis; positive regulation of caspase activity; negative regulation of transport; induction of apoptosis by oxidative stress; positive regulation of stress-activated MAPK cascade