Typical Standard Curve/Testing Data
Human Chloride intracellular channel protein 2 (CLIC2) ELISA Kit | CLIC2 elisa kit
Human Chloride intracellular channel protein 2 (CLIC2) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: CLIC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLIC2 antibody and an CLIC2-HRP conjugate. The assay sample and buffer are incubated together with CLIC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLIC2 concentration since CLIC2 from samples and CLIC2-HRP conjugate compete for the anti-CLIC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLIC2 from the sample, fewer sites are left to bind CLIC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLIC2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a chloride intracellular channel protein. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. This protein may play a role in inhibiting the function of ryanodine receptor 2. A mutation in this gene is the cause of X-linked mental retardation-32. [provided by RefSeq, Aug 2013]
Uniprot Description
CLIC2: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Modulates the activity of RYR2 and inhibits calcium influx. Belongs to the chloride channel CLIC family.
Protein type: Membrane protein, integral; Channel, chloride
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cytoplasm; intracellular; nucleus
Molecular Function: protein binding; chloride channel activity; glutathione transferase activity; voltage-gated ion channel activity; glutathione peroxidase activity
Biological Process: transport; positive regulation of binding; signal transduction
Disease: Mental Retardation, X-linked, Syndromic 32
Research Articles on CLIC2
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Product Notes
The Human CLIC2 clic2 (Catalog #AAA7203397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7203397 ELISA Kit recognizes Human CLIC2. It is sometimes possible for the material contained within the vial of "Chloride intracellular channel protein 2 (CLIC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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