Rabbit TOC33 Polyclonal Antibody | anti-TOC33 antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TOC33 Polyclonal Antibody

• Gene Names: TOC33; ATTOC33; PLASTID PROTEIN IMPORT 1; PPI1; T6A9.32; TOC33; translocon at the outer envelope membrane of chloroplasts 33
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: TOC33; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TOC33 Polyclonal Antibody; TOC33 Antibody; Translocase of chloroplast 33; chloroplastic; AtToc33; EC 3.6.5.-; 33kDa chloroplast outer envelope protein; Plastid protein import 1; TOC33 PPI1 At1g02280 T7I23.11; anti-TOC33 antibody

• Host: Rabbit
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-TOC33 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Arabidopsis thaliana TOC33 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 186478055
• NCBI GeneID: 839248
• NCBI Accession #: NP_001117215.1
• NCBI GenBank Nucleotide #: NM_001123743.1
• UniProt Accession #: O23680; Q94B42; Q9GDD3
• Molecular Weight: 32,925 Da
• NCBI Official Full Name: translocase of chloroplast 33
• NCBI Official Symbol: TOC33
• NCBI Official Synonym Symbols: ATTOC33; PLASTID PROTEIN IMPORT 1; PPI1; T6A9.32; TOC33; translocon at the outer envelope membrane of chloroplasts 33
• NCBI Protein Information: translocase of chloroplast 33
• UniProt Protein Name: Translocase of chloroplast 33, chloroplastic
• Protein Family: Translocase of chloroplast
• UniProt Gene Name: TOC33
• UniProt Synonym Gene Names: PPI1; AtToc33
• UniProt Entry Name: TOC33_ARATH

NCBI Description

Encodes a GTP-binding GTP-ase. Component of the chloroplast protein import machinery. Required for import of POR B into plastids. Toc33 phosphorylation may not play an important role in vivo.

Uniprot Description

Function: GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Binds GTP, GDP, XTP, but not ATP. Probably specialized in the import of nuclear encoded photosynthetic preproteins from the cytoplasm to the chloroplast, especially during early development stages. Ref.7 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.16 Ref.18 Ref.19 Ref.20

Cofactor: Binds 1 magnesium ion by subunit.

Subunit structure: Homodimer, heterodimer with TOC34 and TOC159, and monomer. The homodimerization and the dimerization with TOC159 require the binding of GTP on Arg-130, and an hypothetical coGAP factor. The dimeric form has a higher GTPase activity than the monomeric form. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). Interacts at least with TOC75-3. Forms large complexes including TOC33, pPORA and OEP161 during pPORA import into plastids at the plastid envelope membrane. Ref.1 Ref.8 Ref.9 Ref.11 Ref.12 Ref.17 Ref.22 Ref.23 Ref.24

Subcellular location: Plastid › chloroplast outer membrane; Single-pass membrane protein. Note: May contain beta barrel transmembrane regions. Ref.1 Ref.12 Ref.13 Ref.15

Tissue specificity: Mostly expressed in seedlings and flowers, and, to a lower extent, in roots, stems, and leaves. Ref.1 Ref.14

Developmental stage: Mostly expressed in photosynthetic tissues undergoing rapid growth. Observed in cotyledons and vascular tissues of hypocotyls of young seedling. In roots, restricted to apical and lateral meristems, and vascular bundles. In stems, mostly detected in the upper part. Expressed in young and middle-aged leaves. In flowers, confined to sepals. Ref.1 Ref.14

Induction: Up-regulated by CIA2 in leaves. Induced in light but repressed in darkness. Ref.6 Ref.14

Post-translational modification: Phosphorylated by a kinase present in the outer envelope of chloroplast. When Ser-181 is phosphorylated, the binding to preprotein, GTP and GDP is inhibited, and thus, GTPase activity is repressed. Ref.9 Ref.18 Ref.20

Disruption phenotype: Plants exhibits a pale yellowish phenotype. Ref.1 Ref.7 Ref.14

Sequence similarities: Belongs to the TOC34 family.

Biophysicochemical propertiesKinetic parameters:KM=290 nM for GDP (at pH 7.4) Ref.9 Ref.21KM=5.7 µM for GTP (at pH 7.6 and 25 degrees Celsius)Vmax=1040 nmol/min/µg enzyme with GTP as substrate (at pH 7.4)