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Rabbit FIS Polyclonal Antibody | anti-FIS antibody

Rabbit anti-Escherichia coli (strain K12) FIS Polyclonal Antibody

Gene Names
fis; ECK3248; JW3229; nbp
Reactivity
E Coli (strain K12)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
FIS; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) FIS Polyclonal Antibody; FIS Antibody; DNA-binding protein Fis; Factor-for-inversion stimulation protein; Hin recombinational enhancer-binding protein; fis b3261 JW3229; anti-FIS antibody
Ordering
Host
Rabbit
Reactivity
E Coli (strain K12)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-FIS antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Escherichia coli (strain K12) fis protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,240 Da
NCBI Official Full Name
global DNA-binding transcriptional dual regulator
NCBI Official Symbol
fis
NCBI Official Synonym Symbols
ECK3248; JW3229; nbp
NCBI Protein Information
global DNA-binding transcriptional dual regulator
UniProt Protein Name
DNA-binding protein Fis
Protein Family
UniProt Gene Name
fis

NCBI Description

fis transcription is supercoiling-dependent. [More information is available at EcoGene: EG10317]. Fis, "factor for inversion stimulation", is a small DNA-binding and bending protein whose main role appears to be the organization and maintenance of nucleoid structure through direct DNA binding and by modulating gyrase and topoisomerase I production , as well as regulation of other proteins that modulate the nucleoid structure, such as CRP, HNS, and HU. [More information is available at EcoCyc: EG10317].

Uniprot Description

Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC. Binds to hundreds of transcriptionally active and inactive AT-rich sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779).

Research Articles on FIS

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Product Notes

The FIS fis (Catalog #AAA7162674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) FIS Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's FIS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the FIS fis for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FIS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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