Rabbit RSP5 Polyclonal Antibody | anti-RSP5 antibody

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast) RSP5 Polyclonal Antibody

• Gene Names: RSP5; MDP1; MUT2; NPI1; SMM1; UBY1
• Reactivity: Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: RSP5; Polyclonal Antibody; Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast) RSP5 Polyclonal Antibody; RSP5 Antibody; E3 ubiquitin-protein ligase RSP5; EC 2.3.2.26; HECT-type E3 ubiquitin transferase RSP5; Reverses SPT-phenotype protein 5; RSP5 MDP1 NPI1 YER125W SYGP-ORF41; anti-RSP5 antibody

• Host: Rabbit
• Reactivity: Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-RSP5 antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RSP5 protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 398364769
• NCBI GeneID: 856862
• NCBI Accession #: NP_011051.3
• NCBI GenBank Nucleotide #: NM_001179015.3
• UniProt Accession #: P39940; D3DM31
• Molecular Weight: 91,816 Da
• NCBI Official Full Name: NEDD4 family E3 ubiquitin-protein ligase
• NCBI Official Symbol: RSP5
• NCBI Official Synonym Symbols: MDP1; MUT2; NPI1; SMM1; UBY1
• NCBI Protein Information: NEDD4 family E3 ubiquitin-protein ligase
• UniProt Protein Name: E3 ubiquitin-protein ligase RSP5
• Protein Family: Flagellar radial spoke protein
• UniProt Gene Name: RSP5
• UniProt Synonym Gene Names: MDP1; NPI1

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner.