Rabbit DHAK Polyclonal Antibody | anti-DHAK antibody

Rabbit anti-Escherichia coli (strain K12) DHAK Polyclonal Antibody

• Gene Names: dhaK; dhaK1; ECK1188; JW5187; ptsD; ycgT
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: DHAK; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) DHAK Polyclonal Antibody; DHAK Antibody; PEP-dependent dihydroxyacetone kinase; dihydroxyacetone-binding subunit DhaK; EC 2.7.1.121; dhaK ycgT b1200 JW5187; anti-DHAK antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-DHAK antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) dhaK protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 162135900
• NCBI GeneID: 945747
• NCBI Accession #: NP_415718.6
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P76015
• Molecular Weight: 38,215 Da
• NCBI Official Full Name: dihydroxyacetone kinase, PTS-dependent, dihydroxyacetone-binding subunit
• NCBI Official Symbol: dhaK
• NCBI Official Synonym Symbols: dhaK1; ECK1188; JW5187; ptsD; ycgT
• NCBI Protein Information: dihydroxyacetone kinase, PTS-dependent, dihydroxyacetone-binding subunit
• UniProt Protein Name: PEP-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK
• Protein Family: DhaKLM operon coactivator
• UniProt Gene Name: dhaK

NCBI Description

DhaK is similar to N-terminal half of single protein DHA kinases. [More information is available at EcoGene: EG13901]. The K subunit is similar to the N-terminal half of ATP-dependent dihydroxyacetone kinase of Citrobacter freundii and eukaryotes. [More information is available at EcoCyc: G6627].

Uniprot Description

Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone via a phosphoryl group transfer from DhaL-ATP (PubMed:15476397). Binds covalently dihydroxyacetone in hemiaminal linkage (PubMed:15476397). DhaK acts also as corepressor of the transcription activator DhaR by binding to the sensor domain of DhaR (PubMed:24440518). In the presence of dihydroxyacetone, DhaL-ADP displaces DhaK and stimulates DhaR activity (PubMed:24440518). In the absence of dihydroxyacetone, DhaL-ADP is converted by the PTS to DhaL-ATP, which does not bind to DhaR (PubMed:24440518).