Rabbit Tfap2a Polyclonal Antibody | anti-Tfap2a antibody

Rabbit anti-Mus musculus (Mouse) Tfap2a Polyclonal Antibody

• Gene Names: Tfap2a; Ap2; AP-2; Ap2tf; Tcfap2a; AP2alpha
• Reactivity: Mus musculus (Mouse)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: Tfap2a; Polyclonal Antibody; Rabbit anti-Mus musculus (Mouse) Tfap2a Polyclonal Antibody; Tfap2a Antibody; Transcription factor AP-2-alpha; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2); Ap2tf Tcfap2a; anti-Tfap2a antibody

• Host: Rabbit
• Reactivity: Mus musculus (Mouse)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-Tfap2a antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Mus musculus Tfap2a protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 171846238
• NCBI GeneID: 21418
• NCBI Accession #: NP_001116420.1
• NCBI GenBank Nucleotide #: NM_001122948.1
• UniProt Accession #: P34056; Q60740; Q60741; Q60742; Q60743; Q62067; Q62068; Q62069; Q91VX0; Q9CRY4
• Molecular Weight: 47,971 Da
• NCBI Official Full Name: transcription factor AP-2-alpha isoform b
• NCBI Official Synonym Full Names: transcription factor AP-2, alpha
• NCBI Official Symbol: Tfap2a
• NCBI Official Synonym Symbols: Ap2; AP-2; Ap2tf; Tcfap2a; AP2alpha
• NCBI Protein Information: transcription factor AP-2-alpha; Ap-2 (a); AP2-alpha; AP-2 alpha; activator protein 2; AP-2 transcription factor; activating enhancer-binding protein 2 alpha; activating enhancer-binding protein 2-alpha
• UniProt Protein Name: Transcription factor AP-2-alpha
• Protein Family: Transcription factor
• UniProt Gene Name: Tfap2a
• UniProt Synonym Gene Names: Ap2tf; Tcfap2a; AP2-alpha; AP-2
• UniProt Entry Name: AP2A_MOUSE

NCBI Description

This gene is a member of the activator protein 2 (AP-2) transcription factor family. The protein encoded by this gene can act as both an activator and repressor of gene transcription, and plays an important role in early embryogenesis, specifically in cranial development. This protein forms both homodimers and heterodimers, and binds to a GC-rich consensus sequence found in some promoters and enhancers. Disruption of this gene causes perinatal death, with neural tube, craniofacial, and limb mesenchyme defects. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Sep 2014]

Uniprot Description

AP-2 alpha: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; Golgi apparatus; centrosome; transcription factor complex; intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: embryonic forelimb morphogenesis; negative regulation of epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; skin development; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; forelimb morphogenesis; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; regulation of transcription, DNA-dependent; oculomotor nerve formation; regulation of cell differentiation; positive regulation of neuron apoptosis; epidermis morphogenesis; negative regulation of neuron apoptosis; kidney development; inner ear morphogenesis; trigeminal nerve development; anterior neuropore closure; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; regulation of transcription from RNA polymerase II promoter; embryonic body morphogenesis; skeletal morphogenesis; forebrain neuron development; neural tube closure; lens morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of cell migration