CDK6 recombinant antibody

CDK6 Antibody

Cat. Num. AAA7136676

• Gene Names: CDK6; PLSTIRE
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity-chromatography
• Synonyms: CDK6; Monoclonal Recombinant Antibody; CDK6 Antibody; Cyclin-dependent kinase 6 (EC 2.7.11.22) (Cell division protein kinase 6) (Serine/threonine-protein kinase PLSTIRE); CDKN6; CDK6 recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 8G3
• Purity/Purification: Affinity-chromatography
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, PH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for CDK6 recombinant antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:5000; IP: 1:200-1:1000
• Antibody Type: Recombinant Antibody
• Conjugation: Non-conjugated
• Immunogen: A synthesized peptide derived from human CDK6
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate All lanes: CDK6 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa)

Immunoprecipitation (IP)

(Immunoprecipitating CDK6 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: K562 whole cell lysate?500ug? Lane 3: K562 whole cell lysate (10ug))

Related Product Information for CDK6 recombinant antibody

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).

Product Categories/Family for CDK6 recombinant antibody

Epigenetics and Nuclear Signaling; Cancer; Cell biology

NCBI and Uniprot Product Information

• NCBI GI #: 223718134
• NCBI GeneID: 1021
• NCBI Accession #: NP_001138778.1
• NCBI GenBank Nucleotide #: NM_001145306.1
• UniProt Accession #: Q00534
• Molecular Weight: 326
• NCBI Official Full Name: cyclin-dependent kinase 6
• NCBI Official Synonym Full Names: cyclin-dependent kinase 6
• NCBI Official Symbol: CDK6
• NCBI Official Synonym Symbols: PLSTIRE
• NCBI Protein Information: cyclin-dependent kinase 6; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE
• UniProt Protein Name: Cyclin-dependent kinase 6
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK6
• UniProt Synonym Gene Names: CDKN6
• UniProt Entry Name: CDK6_HUMAN