Vaccinia virus Cell surface-binding protein (D8L) Recombinant Protein | D8L recombinant protein

Recombinant Vaccinia virus Cell surface-binding protein (D8L)

Cat. Num. AAA7135820

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Vaccinia virus Cell surface-binding protein (D8L); Recombinant Vaccinia virus Cell surface-binding protein (D8L); Carbonic anhydrase homolog; D8L recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized powder; If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
• Sequence Positions: 1-304aa; Full Length
• Sequence: MPQQLSPINIETKKAISNARLKPLDIHYNESKPTTIQNTGKLVRINFKGGYISGGFLPNEYVLSSLHIYWGKEDDYGSNHLIDVYKYSGEINLVHWNKKKYSSYEEAKKHDDGLIIISIFLQVSDHKNVYFQKIVNQLDSIRSANTSAPFDSVFYLDNLLPSTLDYFTYLGTTIKHSADAVWIIFPTPINIHSDQLSKFRTLLSSSNHDGKPYYITENYRNPYKLNDDTQVYYSGEIIRAATTSPARENYFMRWLSDLRETCFSYYQKYIEGNKTFAIIAIVFVFILTAILFLMSRRYSREKQN
• Sequence Length: 304

• Species: Vaccinia virus (strain Copenhagen) (VACV)
• Tag: N-terminal 6xHis-tagged
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. ; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.; The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for D8L recombinant protein

Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

References

"Appendix to 'The complete DNA sequence of vaccinia virus'."
Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.Virology 179:517-563(1990)

NCBI and Uniprot Product Information

• NCBI GI #: 115474
• UniProt Accession #: P20508
• Molecular Weight: 41.3kDa
• NCBI Official Full Name: Cell surface-binding protein
• UniProt Protein Name: Cell surface-binding protein
• Protein Family: Cell surface-binding protein
• UniProt Entry Name: CAHH_VACCC

Uniprot Description

Function: Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell. Ref.3

Subunit structure: Homodimer; disulfide-linked.

Subcellular location: Virion membrane

Potential. Note: Component of the mature virion (MV) membrane

Potential.

Induction: Expressed in the late phase of the viral replicative cycle.

Post-translational modification: Apparently non-glycosylated.

Sequence similarities: Belongs to the alpha-carbonic anhydrase family.