Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with MBS7134135 at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Rabbit anti-Human WDR59 Polyclonal Antibody | anti-WDR59 antibody
WDR59 Antibody
Gene Names
WDR59; FP977
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G Purified
Synonyms
WDR59; Polyclonal Antibody; WDR59 Antibody; GATOR complex protein WDR59 (WD repeat-containing protein 59); KIAA1923; anti-WDR59 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Applicable Applications for anti-WDR59 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human GATOR complex protein WDR59 protein (619-862AA)
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with MBS7134135 at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of U251 cells with MBS7134135 at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Western Blot (WB)
(Western BlotPositive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysateAll lanes: WDR59 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 110, 65, 63, 47 kDaObserved band size: 110 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysateAll lanes: WDR59 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 110, 65, 63, 47 kDaObserved band size: 110 kDa)
Product Categories/Family for anti-WDR59 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,793 Da
NCBI Official Full Name
WD repeat-containing protein 59
NCBI Official Synonym Full Names
WD repeat domain 59
NCBI Official Symbol
WDR59
NCBI Official Synonym Symbols
FP977
NCBI Protein Information
WD repeat-containing protein 59
UniProt Protein Name
WD repeat-containing protein 59
Protein Family
UniProt Gene Name
WDR59
UniProt Synonym Gene Names
KIAA1923
UniProt Entry Name
WDR59_HUMAN
Similar Products
Product Notes
The WDR59 wdr59 (Catalog #AAA7134135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WDR59 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR59 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:1000-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the WDR59 wdr59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR59, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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