Mouse Filamin C Gamma ELISA Kit | FLNC elisa kit

Mouse Filamin C Gamma ELISA Kit

Cat. Num. AAA71335

• Gene Names: FLNC; ABPA; ABPL; FLN2; MFM5; MPD4; ABP-280; ABP280A
• Reactivity: Mouse
• Synonyms: Filamin C Gamma; Mouse Filamin C Gamma ELISA Kit; FLNC elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse FLNC and analogues was observed.
• Sequence Length: 2725

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated FLNC concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse FLNC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FLNC and FLNC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FLNC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FLNC elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated FLNC concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse FLNC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FLNC and FLNC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FLNC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 254763419
• NCBI GeneID: 2318
• UniProt Accession #: Q14315; O95303; Q07985; Q9NS12; Q9NYE5; Q9UMR8; Q9Y503; B2ZZ88
• Molecular Weight: 291,022 Da
• NCBI Official Full Name: Filamin-C
• NCBI Official Synonym Full Names: filamin C, gamma
• NCBI Official Symbol: FLNC
• NCBI Official Synonym Symbols: ABPA; ABPL; FLN2; MFM5; MPD4; ABP-280; ABP280A
• NCBI Protein Information: filamin-C; filamin-2; ABP-280-like protein; ABP-L, gamma filamin; actin binding protein 280
• UniProt Protein Name: Filamin-C
• Protein Family: Filamin
• UniProt Gene Name: FLNC
• UniProt Synonym Gene Names: ABPL; FLN2; FLN-C; FLNc
• UniProt Entry Name: FLNC_HUMAN

NCBI Description

This gene encodes one of three related filamin genes, specifically gamma filamin. These filamin proteins crosslink actin filaments into orthogonal networks in cortical cytoplasm and participate in the anchoring of membrane proteins for the actin cytoskeleton. Three functional domains exist in filamin: an N-terminal filamentous actin-binding domain, a C-terminal self-association domain, and a membrane glycoprotein-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FLNC: Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross- linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers. Defects in FLNC are the cause of myopathy myofibrillar type 5 (MFM5). A neuromuscular disorder, usually with an adult onset, characterized by focal myofibrillar destruction and pathological cytoplasmic protein aggregations, and clinical features of a limb-girdle myopathy. Defects in FLNC are the cause of myopathy distal type 4 (MPD4). MPD4 is a slowly progressive muscular disorder characterized by distal muscle weakness and atrophy affecting the upper and lower limbs. Onset occurs around the third to fourth decades of life, and patients remain ambulatory even after long disease duration. Muscle biopsy shows non-specific changes with no evidence of rods, necrosis, or inflammation. Belongs to the filamin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q32-q35

Cellular Component: focal adhesion; costamere; cytoskeleton; cytoplasm; plasma membrane; Z disc; cytosol; sarcoplasm; sarcolemma

Molecular Function: actin filament binding; protein binding; cytoskeletal protein binding; ankyrin binding

Biological Process: muscle fiber development

Disease: Myopathy, Myofibrillar, 5; Myopathy, Distal, 4