Rabbit anti-Human RUNX3 Polyclonal Antibody | anti-RUNX3 antibody

RUNX3 Antibody

Cat. Num. AAA7131848

• Gene Names: RUNX3; AML2; CBFA3; PEBP2aC
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: RUNX3; Polyclonal Antibody; RUNX3 Antibody; Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; AML2; CBF-alpha-3; CBFA3; anti-RUNX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-RUNX3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:3000
• Immunogen: Synthesized peptide derived from Internal of human RUNX3.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from 293/HeLa cells, using RUNX3 antibody. )

Product Categories/Family for anti-RUNX3 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 72534652
• NCBI GeneID: 864
• NCBI Accession #: NP_001026850.1
• NCBI GenBank Nucleotide #: NM_001031680.2
• UniProt Accession #: Q13761; Q12969; Q13760; B1AJV5
• Molecular Weight: 44,356 Da
• NCBI Official Full Name: runt-related transcription factor 3 isoform 1
• NCBI Official Synonym Full Names: runt-related transcription factor 3
• NCBI Official Symbol: RUNX3
• NCBI Official Synonym Symbols: AML2; CBFA3; PEBP2aC
• NCBI Protein Information: runt-related transcription factor 3; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; oncogene AML-2; transcription factor AML2; acute myeloid leukemia gene 2; acute myeloid leukemia 2 protein; core-binding factor subunit alpha-3; SL
• UniProt Protein Name: Runt-related transcription factor 3
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: RUNX3
• UniProt Synonym Gene Names: AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C
• UniProt Entry Name: RUNX3_HUMAN

NCBI Description

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; transcription factor activity; ATP binding

Biological Process: peripheral nervous system neuron development; transcription from RNA polymerase II promoter; axon guidance; hair follicle morphogenesis; regulation of transcription, DNA-dependent; cell maturation; interferon-gamma production; chondrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; negative regulation of epithelial cell proliferation; negative regulation of cell cycle