Rabbit WWTR1 Polyclonal Antibody | anti-WWTR1 antibody

WWTR1 Antibody

Cat. Num. AAA7130525

• Gene Names: WWTR1; TAZ
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: WWTR1; Polyclonal Antibody; WWTR1 Antibody; anti-WWTR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Liquid; Supplied at 1.0mg/mL In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-WWTR1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Peptide sequence around aa.386~390 (V-E-S-A-L) derived from Human TAZ.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extract from JK and Hela cells using TAZ Antibody )

Product Categories/Family for anti-WWTR1 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 270132693
• NCBI GeneID: 25937
• NCBI Accession #: NP_001161750.1
• NCBI GenBank Nucleotide #: NM_001168278.1
• UniProt Accession #: Q9GZV5; Q8N3P2; Q9Y3W6; D3DNH7
• Molecular Weight: 44,101 Da
• NCBI Official Full Name: WW domain-containing transcription regulator protein 1
• NCBI Official Synonym Full Names: WW domain containing transcription regulator 1
• NCBI Official Symbol: WWTR1
• NCBI Official Synonym Symbols: TAZ
• NCBI Protein Information: WW domain-containing transcription regulator protein 1; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ-binding motif
• UniProt Protein Name: WW domain-containing transcription regulator protein 1
• Protein Family: WW domain-containing transcription regulator protein
• UniProt Gene Name: WWTR1
• UniProt Synonym Gene Names: TAZ
• UniProt Entry Name: WWTR1_HUMAN

Uniprot Description

TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.

Protein type: Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3q23-q24

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; transcription coactivator activity; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; transcription, DNA-dependent; stem cell division; multicellular organism growth; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; glomerulus development; osteoblast differentiation; mesenchymal cell differentiation; regulation of transcription, DNA-dependent; negative regulation of protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; negative regulation of protein kinase activity; gene expression; positive regulation of transcription from RNA polymerase II promoter