Rabbit TBX5 Polyclonal Antibody | anti-TBX5 antibody

TBX5 Antibody

Cat. Num. AAA7129290

• Gene Names: TBX5; HOS
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: TBX5; Polyclonal Antibody; TBX5 Antibody; T-box 5; anti-TBX5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol

• Applicable Applications for anti-TBX5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000-1:5000; WB: 1:200-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human TBX5
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using MBS7129290(TBX5 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using MBS7129290(TBX5 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200))

SDS-Page

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse kidney tissue, Primary antibody: MBS7129290(TBX5 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes)

Product Categories/Family for anti-TBX5 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 12644474
• NCBI GeneID: 6910
• UniProt Accession #: Q99593; O15301; Q96TB0; Q9Y4I2; A6ND77
• Molecular Weight: 518
• NCBI Official Full Name: T-box transcription factor TBX5
• NCBI Official Synonym Full Names: T-box 5
• NCBI Official Symbol: TBX5
• NCBI Official Synonym Symbols: HOS
• NCBI Protein Information: T-box transcription factor TBX5; T-box protein 5
• UniProt Protein Name: T-box transcription factor TBX5
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX5
• UniProt Synonym Gene Names: T-box protein 5
• UniProt Entry Name: TBX5_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX5: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX5 are the cause of Holt-Oram syndrome (HOS). HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; pericardium development; transcription initiation from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; pattern specification process; cardiac muscle cell differentiation; forelimb morphogenesis; negative regulation of cell proliferation; cell-cell signaling; apoptotic nuclear changes; negative regulation of cardiac muscle cell proliferation; positive regulation of cardioblast differentiation; morphogenesis of an epithelium; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell migration; lung development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation

Disease: Holt-oram Syndrome