Rabbit anti-Human, Mouse MCM4 Polyclonal Antibody | anti-MCM4 antibody

MCM4 Antibody

Cat. Num. AAA7127489

• Gene Names: MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purification
• Synonyms: MCM4; Polyclonal Antibody; MCM4 Antibody; Minichromosome maintenance complex component 4; anti-MCM4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol

• Applicable Applications for anti-MCM4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:5000; WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human MCM4
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using MBS7127489(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)-Paraffin

(The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MBS7127489(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200))

SDS-Page

(Gel: 8%SDS-PAGE, Lysate: 50 ug, Lane 1-3: NIH/3T3 cells, Hela cells, K562 cells, Primary antibody: MBS7127489(MCM4 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)

Product Categories/Family for anti-MCM4 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 33469919
• NCBI GeneID: 4173
• NCBI Accession #: NP_005905.2
• NCBI GenBank Nucleotide #: NM_005914.3
• UniProt Accession #: P33991; Q8NEH1; Q99658
• Molecular Weight: 96,558 Da
• NCBI Official Full Name: DNA replication licensing factor MCM4
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 4
• NCBI Official Symbol: MCM4
• NCBI Official Synonym Symbols: NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
• NCBI Protein Information: DNA replication licensing factor MCM4; CDC21 homolog; minichromosome maintenance deficient 4; homolog of S. pombe cell devision cycle 21
• UniProt Protein Name: DNA replication licensing factor MCM4
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM4
• UniProt Synonym Gene Names: CDC21
• UniProt Entry Name: MCM4_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex.

Protein type: EC 3.6.4.12; DNA replication

Chromosomal Location of Human Ortholog: 8q11.2

Cellular Component: nucleoplasm; membrane; MCM complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; single-stranded DNA binding; ATP binding

Biological Process: DNA unwinding during replication; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle

Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect