Rabbit anti-Human DDX54 Polyclonal Antibody | anti-DDX54 antibody

DDX54 Antibody

Cat. Num. AAA7116227

• Gene Names: DDX54; DP97
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: DDX54; Polyclonal Antibody; DDX54 Antibody; ATP-dependent RNA helicase DDX54; ATP-dependent RNA helicase DP97; DEAD box RNA helicase 97 kDa; DEAD box protein 54; anti-DDX54 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; In Phosphate Buffered Saline, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.

• Applicable Applications for anti-DDX54 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:10000
• Immunogen: Synthesized peptide derived from the Internal region of human DDX54.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot analysis of Jurkat cells using DDX54 Polyclonal Antibody)

Product Categories/Family for anti-DDX54 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 164419743
• NCBI GeneID: 79039
• NCBI Accession #: NP_001104792.1
• NCBI GenBank Nucleotide #: NM_001111322.1
• UniProt Accession #: Q8TDD1; Q86YT8; Q9BRZ1
• Molecular Weight: 98,595 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DDX54 isoform 1
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
• NCBI Official Symbol: DDX54
• NCBI Official Synonym Symbols: DP97
• NCBI Protein Information: ATP-dependent RNA helicase DDX54; DEAD box protein 54; DEAD box helicase 97 KDa; DEAD box RNA helicase 97 kDa; ATP-dependent RNA helicase DP97
• UniProt Protein Name: ATP-dependent RNA helicase DDX54
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX54
• UniProt Entry Name: DDX54_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX54: Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors. Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 3.6.4.13; Helicase; RNA processing; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: estrogen receptor binding; transcription corepressor activity; ATP binding; ATP-dependent RNA helicase activity; receptor binding

Biological Process: RNA processing; estrogen receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA metabolic process