Cartilage Intermediate Layer Protein 1 (CILP) Recombinant Protein | CILP recombinant protein

Recombinant Human Cartilage Intermediate Layer Protein 1 (CILP), Partial

Cat. Num. AAA7115190

• Gene Names: CILP; CILP-1; HsT18872
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Cartilage Intermediate Layer Protein 1 (CILP); Recombinant Human Cartilage Intermediate Layer Protein 1 (CILP); Partial; Cartilage intermediate-layer protein; CILP recombinant protein

• Host: Baculovirus
• Specificity: Specifically expressed in cartilage. Localizes in the intermediates layer of articular cartilage but neither in the superficial nor in the deepest regions. Specifically and highly expressed in intervertebral disk tissue. Expression increases with aging in hip articular cartilage. Overexpressed in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease (CPPD). Expression in intervertebral disk tissue from individuals with lumbar disk disease increases as disk degeneration progresses.
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 725-1184aa; Partial
• Sequence: EDRTFLVGNLEIRERRLFNLDVPESRRCFVKVRAYRSERFLPSEQIQGVVISVINLEPRTGFLSNPRAWGRFDSVITGPNGACVPAFCDDQSPDAYSAYVLASLAGEELQAVESSPKFNPNAIGVPQPYLNKLNYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVRSTRDRDQPNVSAACLEFKCSGMLYDQDRVDRTLVKVIPQGSCRRASVNPMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCVERQVGRQSAFQYLQSTPAQSPAAGTVQGRVPSRRQQRASRGGQRQGGVVASLRFPRVAQQPLIN
• Sequence Length: 1184

• Species: Human
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Subcellular Location: Secreted, Extracellular Space, Extracellular Matrix
• Production Note: Special Offer: The Baculovirus host-expressed protein is manufactured from a stock plasmid containing the protein gene. Baculovirushost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Baculovirus host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Baculovirus host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for CILP recombinant protein

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.

Product Categories/Family for CILP recombinant protein

Immunology

References

"The human CILP gene: exon/intron organization and chromosomal mapping." Lorenzo P., Aman P., Sommarin Y., Heinegaerd D. Matrix Biol. 18:445-454(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 192449445
• NCBI GeneID: 8483
• NCBI Accession #: NP_003604.3
• NCBI GenBank Nucleotide #: NM_003613.3
• UniProt Accession #: O75339
• Molecular Weight: 55.7 kDa
• NCBI Official Full Name: cartilage intermediate layer protein 1 preproprotein
• NCBI Official Synonym Full Names: cartilage intermediate layer protein
• NCBI Official Symbol: CILP
• NCBI Official Synonym Symbols: CILP-1; HsT18872
• NCBI Protein Information: cartilage intermediate layer protein 1
• UniProt Protein Name: Cartilage intermediate layer protein 1
• Protein Family: Cartilage intermediate layer protein
• UniProt Gene Name: CILP
• UniProt Entry Name: CILP1_HUMAN

NCBI Description

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]