Rabbit anti-Human HOOK3 Polyclonal Antibody | anti-HOOK3 antibody

HOOK3 Antibody

Cat. Num. AAA7114134

• Gene Names: HOOK3; HK3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >95%; Protein G Purified
• Synonyms: HOOK3; Polyclonal Antibody; HOOK3 Antibody; Protein Hook homolog 3; h-hook3; hHK3; anti-HOOK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 718

• Applicable Applications for anti-HOOK3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:5000; IHC: 1:200-1:500; IP: 1:200-1:2000
• Immunogen Species: Human
• Immunogen: Recombinant Human Protein Hook homolog 3 protein (357-455AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: 293 whole cell lysateAll lanes: HOOK3 antibody at 4.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 84 kDaObserved band size: 84 kDa)

Immunohistochemistry (IHC)

(IHC image of CSB-PA769790LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunoprecipitation (IP)

(Immunoprecipitating HOOK3 in 293 whole cell lysateLane 1: Rabbit control IgG instead of CSB-PA769790LA01HU in 293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-PA769790LA01HU (6ug) + 293 whole cell lysate (500ug)Lane 3: 293 whole cell lysate (20ug))

Product Categories/Family for anti-HOOK3 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 14165274
• NCBI GeneID: 84376
• NCBI Accession #: NP_115786.1
• NCBI GenBank Nucleotide #: NM_032410.4
• UniProt Accession #: Q86VS8; Q8NBH0; Q9BY13; D3DSY8
• Molecular Weight: 83,126 Da
• NCBI Official Full Name: protein Hook homolog 3
• NCBI Official Synonym Full Names: hook microtubule tethering protein 3
• NCBI Official Symbol: HOOK3
• NCBI Official Synonym Symbols: HK3
• NCBI Protein Information: protein Hook homolog 3
• UniProt Protein Name: Protein Hook homolog 3
• Protein Family: Protein Hook
• UniProt Gene Name: HOOK3
• UniProt Synonym Gene Names: h-hook3; hHK3

NCBI Description

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM, Apr 2004]

Uniprot Description

Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).