Human WAP four-disulfide core domain 2 ELISA Kit | WFDC2 elisa kit

Human epididymal protein 4, HE4 ELISA Kit

Cat. Num. AAA706042

• Gene Names: WFDC2; HE4; WAP5; EDDM4; dJ461P17.6
• Reactivity: Human
• Synonyms: WAP four-disulfide core domain 2; Human epididymal protein 4; HE4 ELISA Kit; Human epididymal protein 4 (HE4) ELISA kit; EDDM4; HE4; MGC57529; WAP5; dJ461P17.6; WAP domain containing protein HE4-V4; epididymal protein 4; epididymal secretory protein E4; epididymis-specific; whey-acidic protein type; four-disulfide core; m; WFDC2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human WFDC2. No significant cross-reactivity or interference between Human WFDC2 and analogues was observed.
• Sequence Length: 124

• Samples: Serum, plasma, cell culture supernates, ascitic fluid, tissue homogenates, Cell lysates
• Detection Range: 6 pmol/ml-120 pmol/ml
• Sensitivity: 1.52 pmol/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WFDC2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with Avidin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody and Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for HE4 and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HE4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56699495
• NCBI GeneID: 10406
• NCBI Accession #: NP_006094.3
• NCBI GenBank Nucleotide #: NM_006103.3
• UniProt Accession #: Q14508; Q6IB27; Q8WXV9; Q8WXW0; Q8WXW1; Q8WXW2; Q96KJ1; A2A2A5; A2A2A6; A6PVD5
• Molecular Weight: 12,993 Da
• NCBI Official Full Name: WAP four-disulfide core domain protein 2
• NCBI Official Synonym Full Names: WAP four-disulfide core domain 2
• NCBI Official Symbol: WFDC2
• NCBI Official Synonym Symbols: HE4; WAP5; EDDM4; dJ461P17.6
• NCBI Protein Information: WAP four-disulfide core domain protein 2; epididymal protein 4; epididymal secretory protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; major epididymis-specific protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core
• UniProt Protein Name: WAP four-disulfide core domain protein 2
• Protein Family: WAP four-disulfide core domain protein
• UniProt Gene Name: WFDC2
• UniProt Synonym Gene Names: HE4; WAP5
• UniProt Entry Name: WFDC2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Broad range protease inhibitor.1 PublicationManual assertion based on experiment in:Ref.10