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Nitrofurazone (SEM) ELISA Kit | SEM elisa kit

Nitrofurazone (SEM) ELISA Kit

Synonyms
Nitrofurazone (SEM); Nitrofurazone (SEM) ELISA Kit; SEM elisa kit
Ordering
For Research Use Only!
Sequence Length
239
Samples
Milk, honey, tissue(fish, shrimp, chicken, pork, liver).
Assay Type
Competitive
Detection Range
0. 1 ppb -8.1 ppb
Sensitivity
Typically less than 0.1ppb
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for SEM elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nitrofurazone antigen. Standards or samples are added to the appropriate microtiter plate wells with Nitrofurazone specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Nitrofurazone and Nitrofurazone in standards or samples with the Nitrofurazone special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Nitrofurazone in the standards or samples. The color development is stopped and the intensity of the color is measured.

Background/Introduction: Nitrofurans are synthetic broad-spectrum antibiotics, widely used in animal production for its excellent antibacterial and pharmacokinetic properties. They had been also used as growth promoters in the pig production, the poultry and fish sector. In long term studies with experimental animals the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics. This has led to a prohibition of Nitrofurans for the treatment of animals used for food production. Current methods of detection Nitrofurazone, such as LC-UV, LC-MS and LC-MS/MS, are with sample preparation complex, expensive, difficult and time-consuming. While using the Nitrofurazone ELISA kit is both rapidly and accurately detect Nitrofurazone residues in samples This kit is a detection product developed based on ELISA technology, with simple operation and a sensitivity of 0.1 ppb, and linear range from 0.1 ppb to 8.1 ppb.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
27,372 Da
NCBI Official Full Name
SEM
UniProt Protein Name
SEM
UniProt Gene Name
sem
UniProt Entry Name
Q9EZM7_STAAU

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Product Notes

The SEM sem (Catalog #AAA705345) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nitrofurazone (SEM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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