Human chemokine (C-C motif) ligand 27 ELISA Kit | CCL27 elisa kit

Human cutaneous lymphocyte-associated antigen, CLA ELISA Kit

Cat. Num. AAA704198

• Gene Names: CCL27; ALP; ILC; CTAK; CTACK; PESKY; ESKINE; SCYA27
• Reactivity: Human
• Synonyms: chemokine (C-C motif) ligand 27; Human cutaneous lymphocyte-associated antigen; CLA ELISA Kit; ALP; CTACK; CTAK; ESKINE; ILC; PESKY; SCYA27; CC chemokine ILC; IL-11 Ralpha-locus chemokine; cutaneous T-cell attracting chemokine; skinkine; small inducible cytokine A27; small inducible cytokine subfa; CLA; CCL27 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CCL27. No significant cross-reactivity or interference between Human CCL27 and analogues was observed.
• Sequence Length: 112

• Assay Type: Sandwich
• Detection Range: 0.313ng/ml-20 ng/ml
• Sensitivity: 0.043ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cytokine
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL27 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5730035
• NCBI GeneID: 10850
• NCBI Accession #: NP_006655.1
• NCBI GenBank Nucleotide #: NM_006664.2
• UniProt Accession #: Q9Y4X3
• Molecular Weight: 12,618 Da
• NCBI Official Full Name: C-C motif chemokine 27
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 27
• NCBI Official Symbol: CCL27
• NCBI Official Synonym Symbols: ALP; ILC; CTAK; CTACK; PESKY; ESKINE; SCYA27
• NCBI Protein Information: C-C motif chemokine 27; skinkine; CC chemokine ILC; IL-11 Ralpha-locus chemokine; small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; cutaneous T-cell attracting chemokine; cutaneous T-cell-attracting chemokine; small inducible cytokine subfamily A (Cys-Cys), member 27
• UniProt Protein Name: C-C motif chemokine 27
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL27
• UniProt Synonym Gene Names: ILC; SCYA27; CTACK
• UniProt Entry Name: CCL27_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.