B4GALT3 antibody pair

B4GALT3 Antibody Pair

Cat. Num. AAA7041719

• Gene Names: B4GALT3; beta4Gal-T3
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: B4GALT3; Polyclonal Antibody Pair; B4GALT3 Antibody Pair; Beta-1; 4-galactosyltransferase 3; UDP-Gal:beta-GlcNAc beta-1; 4-galactosyltransferase 3 UDP-galactose:beta-N-acetylglucosamine beta-1; B4GALT3 antibody pair

• Host: Capture: Rabbit; Detection: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Capture: IgG; Detection: IgG
• Form/Format: Liquid
• Concentration: The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
• Sequence Length: 393

• Applicable Applications for B4GALT3 antibody pair: Sandwich ELISA (EIA)
• Application Notes: We recommend using the capture antibody at a concentration of 0.1ug/ml and the detection antibody at a concentration of 0.025ug/ml.
• Immunogen Species: Human
• Conjugation: Capture: Non-conjugated; Detection: Biotin conjugated
• Recommended Standard: HT-29 cell; MCF-7 cell; RPMI-8226 cell; (Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
• Storage Buffer: Capture: 50% Glycerol, 0.01M PBS, PH 7.4; Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Other Note: Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

• NCBI GI #: 315467844
• NCBI GeneID: 8703
• NCBI Accession #: NP_001186803.1
• NCBI GenBank Nucleotide #: NM_001199874.1
• Molecular Weight: 15,654 Da
• NCBI Official Full Name: beta-1,4-galactosyltransferase 3
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 3
• NCBI Official Symbol: B4GALT3
• NCBI Official Synonym Symbols: beta4Gal-T3
• NCBI Protein Information: beta-1,4-galactosyltransferase 3
• UniProt Protein Name: Beta-1,4-galactosyltransferase 3
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT3
• UniProt Synonym Gene Names: Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3
• UniProt Entry Name: B4GT3_HUMAN

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

B4GALT3: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Belongs to the glycosyltransferase 7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.90; Glycan Metabolism - keratan sulfate biosynthesis; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; EC 2.4.1.38

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; Golgi apparatus; Golgi membrane; integral to membrane

Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; galactosyltransferase activity; metal ion binding; N-acetyllactosamine synthase activity

Biological Process: keratan sulfate biosynthetic process; protein amino acid glycosylation