Human heat shock 10kDa protein 1 (chaperonin 10) ELISA Kit | HSPE1 elisa kit

Human chaperonin 10, CPN10 ELISA Kit

Cat. Num. AAA703143

• Gene Names: HSPE1; EPF; CPN10; GROES; HSP10
• Reactivity: Human
• Synonyms: heat shock 10kDa protein 1 (chaperonin 10); Human chaperonin 10; CPN10 ELISA Kit; CPN10; GROES; HSP10; chaperonin 10; heat shock 10kD protein 1 (chaperonin 10) ; heat shock 10kDa protein 1; HSPE1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CPN10. No significant cross-reactivity or interference between human CPN10 and analogues was observed.
• Sequence Length: 102

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.16 ng/ml -10 ng/ml.
• Sensitivity: 0.04 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSPE1 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPN10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPN10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPN10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPN10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504523
• NCBI GeneID: 3336
• NCBI Accession #: NP_002148.1
• NCBI GenBank Nucleotide #: NM_002157.2
• UniProt Accession #: P61604; O95421; Q04984; Q53X54; Q9UDH0
• Molecular Weight: 10,932 Da
• NCBI Official Full Name: 10 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 10kDa protein 1
• NCBI Official Symbol: HSPE1
• NCBI Official Synonym Symbols: EPF; CPN10; GROES; HSP10
• NCBI Protein Information: 10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10)
• UniProt Protein Name: 10 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPE1
• UniProt Synonym Gene Names: Hsp10; CPN10; EPF
• UniProt Entry Name: CH10_HUMAN

NCBI Description

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Uniprot Description

Function: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.UniRule annotationHAMAP-Rule:MF_00580