Human alpha-2-macroglobulin ELISA Kit | A2M elisa kit

Human alpha2 macroglobulin, alpha2 MG ELISA Kit

Cat. Num. AAA702999

• Gene Names: A2M; A2MD; CPAMD5; FWP007; S863-7
• Reactivity: Human
• Synonyms: alpha-2-macroglobulin; Human alpha2 macroglobulin; alpha2 MG ELISA Kit; CPAMD5; DKFZp779B086; FWP007; S863-7; ; A2M elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human A2M. No significant cross-reactivity or interference between Human A2M and analogues was observed.
• Sequence Length: 1474

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 1.25 ug/ml-20 ug/ml
• Sensitivity: 1.25 ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for A2M elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to alpha2 MG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated alpha2 MG and incubated. A competitive inhibition reaction is launched between alpha2 MG (Standards or samples) and HRP-conjugated alpha2 MG with the pre-coated antibody specific for alpha2 MG. The more amount of alpha2 MG in samples, the less antibody bound by HRP-conjugated alpha2 MG. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of alpha2 MG in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 66932947
• NCBI GeneID: 2
• NCBI Accession #: NP_000005.2
• NCBI GenBank Nucleotide #: NM_000014.4
• UniProt Accession #: P01023; Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97
• Molecular Weight: 163,291 Da
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2M
• NCBI Official Synonym Symbols: A2MD; CPAMD5; FWP007; S863-7
• NCBI Protein Information: alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2M
• UniProt Synonym Gene Names: CPAMD5; FWP007; Alpha-2-M
• UniProt Entry Name: A2MG_HUMAN

NCBI Description

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.