3-epi-6-deoxocathasterone 23-monooxygenase (ROT3) Recombinant Protein | ROT3 recombinant protein
Recombinant Arabidopsis thaliana 3-epi-6-deoxocathasterone 23-monooxygenase (ROT3)
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
NCBI and Uniprot Product Information
NCBI Description
Encodes a cytochrome P-450 gene that is involved in leaf blade expansion by controlling polar cell expansion in the leaf length direction. Member of the CYP90C CYP450 family. ROT3 was shown to be involved in brassinosteroid biosynthesis, most likely in the conversion step of typhasterol (TY) to castasterone (CS). As 6-deoxo-CS was unable to restore the phenotype of rot3-1, it has been postulated that ROT3 might be specifically involved in the conversion of TY to CS in the C6-oxidation pathway of brassinolide. Recently, CYP90C1 was shown to catalyse the C-23 hydroxylation of several brassinosteroids (the enzyme has a broad specificity for 22-hydroxylated substrates).
Uniprot Description
C-23 hydroxylase involved in brassinosteroids biosynthesis. Converts directly (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6-deoxoteasterone and 6-deoxotyphasterol. These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6-deoxoteasterone. Functionally redundant with CYP90D1.
Research Articles on ROT3
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Product Notes
The ROT3 rot3 (Catalog #AAA7029100) is a Recombinant Protein produced from Cell Free Expression and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-524aa; full length protein. This is a recombinant transmembrane protein expressed in a cell-free expression system. Researchers should empirically determine the suitability of the ROT3 rot3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MQPPASAGLF RSPENLPWPY NYMDYLVAGF LVLTAGILLR PWLWLRLRNS KTKDGDEEED NEEKKKGMIP NGSLGWPVIG ETLNFIACGY SSRPVTFMDK RKSLYGKVFK TNIIGTPIII STDAEVNKVV LQNHGNTFVP AYPKSITELL GENSILSING PHQKRLHTLI GAFLRSPHLK DRITRDIEAS VVLTLASWAQ LPLVHVQDEI KKMTFEILVK VLMSTSPGED MNILKLEFEE FIKGLICIPI KFPGTRLYKS LKAKERLIKM VKKVVEERQV AMTTTSPAND VVDVLLRDGG DSEKQSQPSD FVSGKIVEMM IPGEETMPTA MTLAVKFLSD NPVALAKLVE ENMEMKRRKL ELGEEYKWTD YMSLSFTQNV INETLRMANI INGVWRKALK DVEIKGYLIP KGWCVLASFI SVHMDEDIYD NPYQFDPWRW DRINGSANSS ICFTPFGGGQ RLCPGLELSK LEISIFLHHL VTRYSWTAEE DEIVSFPTVK MKRRLPIRVA TVDDSASPIS LEDH. It is sometimes possible for the material contained within the vial of "3-epi-6-deoxocathasterone 23-monooxygenase (ROT3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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