Adenosine tRNA methylthiotransferase MiaB (miaB) Recombinant Protein | miaB recombinant protein
Recombinant Mycobacterium avium (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Adenosine tRNA methylthiotransferase MiaB (miaB); Recombinant Mycobacterium avium (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; miaB recombinant protein
Host
Cell Free Expression
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
1-715aa; full length protein
Sequence
MTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRA AEGAEVADVVVFNTCAVRENADNKLYGNLSHLAPRKRSNPQMQIAVGGCLAQKDREAVLR RAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQFPSSLPSARESAYAAWVSIS VGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGS DRVLRAMRRSYRAERYLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARF AAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESISLQGNQALVGQTVELLVA TGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGILSHRR TRAGDAHAPAGARAASVSACPPSGRRRPGPTRWMCLMNDDRNHDDPRLGALRTEIEAAER RVAGGIDPGARGFVVSILVFVLLGSFILPHTGDVRGWDVLFGTHDAGAAAVALPSRVFGW LALVFGVGFSTLALVTRRWALAWIALAGTAIAGAAGMLAIWSRQTVPAGHPGPGWGLIVA WITVLVLIYQWARVVWSRTIVQLAAEEQRRRVAAQQQSTTLLDDLPKPEDPAAGT
Sequence Length
Full length protein
Application Notes
This is a recombinant transmembrane protein expressed in a cell-free expression system.
Species
Mycobacterium avium (strain 104)
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Product Categories/Family for miaB recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
77,192 Da
NCBI Official Full Name
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
UniProt Protein Name
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Protein Family
UniProt Gene Name
miaB
UniProt Entry Name
MIAB_MYCA1
Uniprot Description
Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.
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Product Notes
The miaB miab (Catalog #AAA7020349) is a Recombinant Protein produced from Cell Free Expression and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-715aa; full length protein. This is a recombinant transmembrane protein expressed in a cell-free expression system. Researchers should empirically determine the suitability of the miaB miab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTCGFSRADR SPYHGPVTST VARDVSGVRT YQVRTYGCQM NVHDSERLAG LLEAAGYRRA AEGAEVADVV VFNTCAVREN ADNKLYGNLS HLAPRKRSNP QMQIAVGGCL AQKDREAVLR RAPWVDVVFG THNIGSLPTL LERARHNKAA QVEIAEALQQ FPSSLPSARE SAYAAWVSIS VGCNNSCTFC IVPSLRGKEV DRSPDDILAE VRSLVADGVL EVTLLGQNVN AYGVSFADPA LPRDRGAFAR LLRACGEIDG LERVRFTSPH PAEFTDDVIE AMAQTPNVCP ALHMPLQSGS DRVLRAMRRS YRAERYLGII DRVRAAMPHA AITTDLIVGF PGETEEDFAA TLDVVRRARF AAAFTFQYSK RPGTPAAELD GQIPKAVVQE RYERLVELQE SISLQGNQAL VGQTVELLVA TGEGRKDSAT ARMSGRARDG RLVHFAADDR VRPGDLVTTV ITGAAPHHLI ADAGILSHRR TRAGDAHAPA GARAASVSAC PPSGRRRPGP TRWMCLMNDD RNHDDPRLGA LRTEIEAAER RVAGGIDPGA RGFVVSILVF VLLGSFILPH TGDVRGWDVL FGTHDAGAAA VALPSRVFGW LALVFGVGFS TLALVTRRWA LAWIALAGTA IAGAAGMLAI WSRQTVPAGH PGPGWGLIVA WITVLVLIYQ WARVVWSRTI VQLAAEEQRR RVAAQQQSTT LLDDLPKPED PAAGT. It is sometimes possible for the material contained within the vial of "Adenosine tRNA methylthiotransferase MiaB (miaB), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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