Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide ELISA Kit | YWHAB elisa kit

Human 14-3-3 protein beta/alpha, YWHAB ELISA Kit

Cat. Num. AAA701844

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• Reactivity: Human
• Synonyms: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; beta polypeptide; Human 14-3-3 protein beta/alpha; YWHAB ELISA Kit; Human 14-3-3 protein beta/alpha (YWHAB) ELISA kit; GW128; HS1; KCIP-1; 14-3-3 protein beta/alpha; brain protein 14-3-3; beta isoform; protein 1054; protein kinase C inhibitor protein-1; YWHAB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human YWHAB. No significant cross-reactivity or interference between human YWHAB and analogues was observed.
• Sequence Length: 246

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25 ng/ml-400 ng/ml
• Sensitivity: <1.56 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAB elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507949
• NCBI GeneID: 7529
• NCBI Accession #: NP_003395.1
• NCBI GenBank Nucleotide #: NM_003404.4
• UniProt Accession #: P31946; A8K9K2; E1P616
• Molecular Weight: 28,082 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1; HEL-S-1
• NCBI Protein Information: 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; epididymis secretory protein Li 1; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide
• UniProt Protein Name: 14-3-3 protein beta/alpha
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_HUMAN

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.2 PublicationsManual assertion based on experiment in:Ref.13