Human BCL2-associated X protein ELISA Kit | BAX elisa kit

Human Apoptosis regulator BAX ELISA Kit

Cat. Num. AAA701787

• Gene Names: BAX; BCL2L4
• Reactivity: Human
• Synonyms: BCL2-associated X protein; Human Apoptosis regulator BAX ELISA Kit; BCL2L4; apoptosis regulator BAX; BAX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human BAX. No significant cross-reactivity or interference between Human BAX and analogues was observed.
• Sequence Length: 221

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: 0.312 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BAX elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BAX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BAX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BAX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 612149787
• NCBI GeneID: 581
• NCBI Accession #: NP_001278357.1
• NCBI GenBank Nucleotide #: NM_001291428.1
• UniProt Accession #: Q07812; P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1
• Molecular Weight: 21,184 Da
• NCBI Official Full Name: apoptosis regulator BAX isoform 1
• NCBI Official Synonym Full Names: BCL2-associated X protein
• NCBI Official Symbol: BAX
• NCBI Official Synonym Symbols: BCL2L4
• NCBI Protein Information: apoptosis regulator BAX; bcl2-L-4; bcl-2-like protein 4; BCL2-associated X protein omega
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Protein
• UniProt Gene Name: BAX
• UniProt Synonym Gene Names: BCL2L4; Bcl2-L-4
• UniProt Entry Name: BAX_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.6 PublicationsManual assertion based on experiment in:Ref.1