Human coagulation factor X ELISA Kit | F10 elisa kit

Human coagulation factor X, FX ELISA Kit

Cat. Num. AAA701623

• Gene Names: F10; FX; FXA
• Reactivity: Human
• Synonyms: coagulation factor X; Human coagulation factor X; FX ELISA Kit; RP11-98F14.9; FX; FXA; Stuart-Prower factor; factor Xa; prothrombinase; F10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human F10. No significant cross-reactivity or interference between Human F10 and analogues was observed.
• Sequence Length: 488

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F10 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for FX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495.1
• NCBI GenBank Nucleotide #: NM_000504.3
• UniProt Accession #: P00742; Q14340
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor
• UniProt Protein Name: Coagulation factor X
• Protein Family: Coagulation factor
• UniProt Gene Name: F10
• UniProt Entry Name: FA10_HUMAN

NCBI Description

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Catalytic activity: Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.

Enzyme regulation: Inhibited by SERPINA5 and SERPINA10.2 PublicationsManual assertion based on experiment in:Ref.11