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Typical Testing Data/Standard Curve (for reference only)

Human fibroblast growth factor 13 ELISA Kit | FGF13 elisa kit

Human fibroblast growth factor-13, FGF-13 ELISA Kit

Gene Names
FGF13; FGF2; FHF2; FHF-2; FGF-13
Reactivity
Human
Synonyms
fibroblast growth factor 13; Human fibroblast growth factor-13; FGF-13 ELISA Kit; RP6-27P15.3; FGF2; FHF-2; FHF2; fibroblast growth factor homologous factor 2; FGF13 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human FGF-13. No significant cross-reactivity or interference between human FGF-13 and analogues was observed.
Sequence Length
199
Samples
Serum, plasma, cell culture supernates
Assay Type
Sandwich
Detection Range
12.5 pg/ml -800 pg/ml.
Sensitivity
The minimum detectable dose of human FGF-13 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FGF13 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,564 Da
NCBI Official Full Name
fibroblast growth factor 13 isoform 4
NCBI Official Synonym Full Names
fibroblast growth factor 13
NCBI Official Symbol
FGF13
NCBI Official Synonym Symbols
FGF2; FHF2; FHF-2; FGF-13
NCBI Protein Information
fibroblast growth factor 13; fibroblast growth factor homologous factor 2
UniProt Protein Name
Fibroblast growth factor 13
Protein Family
UniProt Gene Name
FGF13
UniProt Synonym Gene Names
FHF2; FGF-13; FHF-2
UniProt Entry Name
FGF13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini. [provided by RefSeq, Nov 2008]

Uniprot Description

Function: Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus.1 PublicationManual assertion based on experiment in:Ref.11

Research Articles on FGF13

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Product Notes

The Human FGF13 fgf13 (Catalog #AAA701587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701587 ELISA Kit recognizes Human FGF13. It is sometimes possible for the material contained within the vial of "fibroblast growth factor 13, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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