Human basigin (Ok blood group) ELISA Kit | BSG elisa kit

Human Cluster of differentiation 147, CD147 ELISA Kit

Cat. Num. AAA701560

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Reactivity: Human
• Synonyms: basigin (Ok blood group); Human Cluster of differentiation 147; CD147 ELISA Kit; CD147 Elisa Kit; 5F7; CD147; EMMPRIN; M6; OK; TCSF; CD147 antigen; M6 leukocyte activation antigen; OK blood group antigen; basigin; collagenase stimulatory factor; extracellular matrix metalloproteinase inducer; BSG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human BSG. No significant cross-reactivity or interference between Human BSG and analogues was observed.
• Sequence Length: 385

• Detection Range: 12.5 pg/ml-800 pg/ml
• Sensitivity: 3.2 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BSG elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD147. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD147 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CD147, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CD147 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38372919
• NCBI GeneID: 682
• NCBI Accession #: NP_001719.2
• NCBI GenBank Nucleotide #: NM_001728.3
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• Molecular Weight: 42,200 Da
• NCBI Official Full Name: basigin isoform 1
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: UNQ6505/PRO21383; EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.1 PublicationManual assertion based on experiment in:Ref.22