Rat tumor protein p53 ELISA Kit | TP53 elisa kit

Rat p53/tumor protein, p53/TP53 ELISA Kit

Cat. Num. AAA700880

• Reactivity: Rat
• Synonyms: tumor protein p53; Rat p53/tumor protein; p53/TP53 ELISA Kit; FLJ92943; LFS1; TRP53; p53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TP53 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat TP53. No significant cross-reactivity or interference between Rat TP53 and analogues was observed.
• Sequence Length: 391

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 12.5 pg/ml-800 pg/ml
• Sensitivity: 3.12 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TP53 elisa kit

Introduction: P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. p53 is important in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. As such, p53 has been described as "the guardian of the genome", the "guardian angel gene", and the "master watchman", referring to its role in conserving stability by preventing genome mutation. The name p53 is in reference to its apparent molecular mass: It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. But, based on calculations from its amino acid residues, p53's mass is actually only 43.7 kDa. This difference is due to the high number of proline residues in the protein, which slow its migration on SDS-PAGE, thus making it appear heavier than it actually is. This effect is observed with p53 from a variety of species, including humans, rodents, frogs, and fish.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to p53/TP53. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for p53/TP53 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain p53/TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of p53/TP53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126722898
• NCBI GeneID: 100009292
• NCBI Accession #: NP_001075873.1
• NCBI GenBank Nucleotide #: NM_001082404.1
• UniProt Accession #: Q95330
• Molecular Weight: 43,435 Da
• NCBI Official Full Name: cellular tumor antigen p53
• NCBI Official Symbol: TP53
• NCBI Protein Information: cellular tumor antigen p53; tumor suppressor p53
• UniProt Protein Name: Cellular tumor antigen p53
• Protein Family: Tumor protein p53-inducible protein
• UniProt Gene Name: TP53
• UniProt Entry Name: P53_RABIT

Uniprot Description

Function: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation

Cofactor: Binds 1 zinc ion per subunit