Rabbit anti-Human ATG4D Polyclonal Antibody | anti-ATG4D antibody

ATG4D Antibody

Cat. Num. AAA7003312

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Reactivity: Human
• Applications: ELISA
• Purity: >95%,Protein G purified
• Synonyms: ATG4D; Polyclonal Antibody; ATG4D Antibody; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; APG4D; AUTL4; anti-ATG4D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 411

• Applicable Applications for anti-ATG4D antibody: ELISA (EIA)
• Immunogen: Recombinant human Cysteine protease ATG4D protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: A549 whole cell lysate,Hela whole cell lysate,SH-SY5Y whole cell lysate,Mouse heart tissueAll lanes: ATG4D antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53,17 KDaObserved band size: 53 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7003312 diluted at 1:700 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7003312 diluted at 1:700 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescent analysis of PC3 cells using MBS7003312 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-ATG4D antibody

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

NCBI and Uniprot Product Information

• NCBI GI #: 528078306
• NCBI GeneID: 84971
• NCBI Accession #: NP_001268433.1
• NCBI GenBank Nucleotide #: NM_001281504.1
• UniProt Accession #: Q86TL0; Q969K0
• Molecular Weight: 16,008 Da
• NCBI Official Full Name: cysteine protease ATG4D isoform 2
• NCBI Official Synonym Full Names: autophagy related 4D cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D
• UniProt Protein Name: Cysteine protease ATG4D
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4
• UniProt Entry Name: ATG4D_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.

Protein type: EC 3.4.22.-; Ubiquitin conjugating system; Protease; Autophagy

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; cytosol; mitochondrial matrix

Molecular Function: cysteine-type endopeptidase activity

Biological Process: apoptosis; autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane